Thermo Fisher Scientific CD11b Monoclonal Antibody (5C6)
CD11b 단백질을 인식하는 Rat 모노클로날 항체(클론 5C6)로, 인간 및 마우스 시료에 반응합니다. IHC, ICC, Flow Cytometry 등 다양한 응용에 적합하며, 저엔도톡신·무보존제 포뮬레이션으로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 1 publication |
| Flow Cytometry (Flow) | 1:50–1:100 | 4 publications |
| Immunoprecipitation (IP) | Assay-dependent | - |
| Neutralization (Neu) | - | 2 publications |
| Functional Assay (Functional) | Assay-dependent | - |
| In vitro Assay (IV) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Bovine, Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5C6 |
| Immunogen | Thioglycollate-elicited Peritoneal Macrophages (TPM) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538033 |
Additional Formats Available:
Product Specific Information
- Low-endotoxin, preservative-free formulation (Endotoxin level: <0.01 EU/µg)
- For FACS analysis: Use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
- Rat anti-Mouse CD11b antibody (clone 5C6) recognizes CD11b, also known as the integrin alpha M chain.
Target Information
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18).
The CD11b/CD18 heterodimeric complex (integrin alpha-M beta-2, Mac-1, CR3) functions as a receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X.
These interactions are critical for cell-cell and cell-matrix adhesion.
CD11b is expressed on:
- Monocytes/macrophages
- Granulocytes
- Activated lymphocytes
- Subsets of NK and dendritic cells
- Microglia in the brain
Expression levels:
- 8% of spleen cells
- 44% of bone marrow cells
- <1% of thymocytes
It is widely used as a microglial marker in nervous tissue. Expression increases during monocyte maturation and varies across tissue macrophages.
Peritoneal macrophages express higher CD11b levels than splenic macrophages.
Dysfunction of CD11b is associated with systemic lupus erythematosus and ITGAM-related susceptibility to autoimmune diseases.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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