Thermo Fisher Scientific 53BP1 Polyclonal Antibody, DyLight 650
DyLight 650 형광 표지된 Thermo Fisher 53BP1 폴리클로날 항체로, 인간 및 생쥐 시료에 반응. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 안정성을 제공.
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Thermo Fisher Scientific 53BP1 Polyclonal Antibody, DyLight 650
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Flow Cytometry (Flow) | 2–5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a portion of human 53BP1 encoded in exon 11, 12, and 19 (Uniprot: Q12888, aa 12–19) |
| Conjugate | DyLight™ 650 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Liquid |
| Concentration | 0.81 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153393 |
Product Specific Information
This antibody reacts with human samples in Western blot and ICC applications, and with mouse samples in ICC applications.
Target Information
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and acts as a substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to sites of double-strand breaks. It may participate in checkpoint signaling during mitosis, enhance TP53-mediated transcriptional activation, and maintain genomic stability through DNA repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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