Thermo Fisher Scientific Phospho-MyoD1 (Ser200) Polyclonal Antibody
Phospho-MyoD1 (Ser200) 폴리클로날 항체로, 사람·마우스·랫에서 반응합니다. Western blot, IHC, ICC/IF, ELISA에 사용 가능하며, KLH 결합 합성 인산화 펩타이드로부터 제작되었습니다. 단백질 A 정제, 1 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human MyoD1 around the phosphorylation site of Ser200 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The Myogenic determination gene (MyoD) was first identified by its ability to convert embryonic mouse fibroblast cells into muscle cells. Forced expression of MyoD (human homolog is myf3) in various normal and neoplastic cells can induce muscle cell conversion or activate muscle-specific genes.
The regulatory domain of MyoD lies within a 70 amino acid region containing a basic DNA-binding motif and a helix-loop-helix (HLH) dimerization motif. Related genes sharing sequence homology include myf5, myogenin (myf4), and myf6 (MRF4/herculin).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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