Thermo Fisher Scientific Phospho-STAT3 (Tyr705) Monoclonal Antibody (LUVNKLA), APC, eBioscience

Thermo Fisher Scientific Phospho-STAT3 (Tyr705) Monoclonal Antibody (LUVNKLA), APC, eBioscience™

Applications

• Flow Cytometry (Intracellular staining)

Tested Dilution

• 5 µL (0.25 µg)/test

Publications

• View 10 publications

Product Specifications

Additional Formats

• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation available

Product Specific Information

This LUVNKLA monoclonal antibody recognizes human and mouse STAT3 phosphorylated at tyrosine 705 (Y705).
STAT3 is a transcription factor activated by cytokine receptor–associated Janus kinases (JAKs). Upon phosphorylation, STAT3 translocates to the nucleus to regulate gene expression involved in cell growth, differentiation, and survival.
STAT3 activation is crucial for Th17 cell differentiation and is implicated in various cancers.

Applications Reported:
Intracellular staining followed by flow cytometric analysis.

Applications Tested:
Pre-titrated for intracellular staining of stimulated human peripheral blood cells.
Use at 5 µL (0.25 µg) per test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.

Staining Protocol:
Use Protocol C: Two-step (Fixation/Methanol).
Protocols A and B are not suitable.
Refer to BestProtocols® – Staining Intracellular Antigens for Flow Cytometry for detailed instructions.

Excitation/Emission:

• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

STAT3 is a member of the STAT family of transcription factors activated by phosphorylation via receptor-associated kinases. It translocates to the nucleus and regulates gene expression in response to cytokines and growth factors such as IFN-α, IFN-γ, EGF, PDGF, and IL-6.
Phosphorylation at Tyr705 by JAK1/JAK2 is essential for dimerization and DNA binding.
STAT3 interacts with coactivators like CREB-binding protein and transcription factors such as c-Jun and CREB.
It plays key roles in cell growth, apoptosis, and immune regulation.
Three alternatively spliced isoforms have been identified.
Deletion of STAT3 in mice is embryonic lethal.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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