Thermo Fisher Scientific PrP Monoclonal Antibody (F89/160.1.5)

Applications

Tested Dilution

Publications

Western Blot (WB)

3-5 µg/mL

-

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

3-5 µg/mL

-

Immunocytochemistry (ICC/IF)

1:200

-

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Deer, Human, Mouse, Sheep

Published species

Hamster, Human, Sheep

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

F89/160.1.5

Immunogen

Synthetic peptide corresponding to residues S(146) R P L I H F G S D Y E D R(159) of bovine PrP. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PrP,uniProtId:P10279-1,ncbiNodeId:9913,antigenRange:146-159,antigenLength:264,antigenImageFileName:MA1-750_PrP_P10279-1_House_mouse.svg,antigenImageFileNamePDP:MA1-750_PrP_P10279-1_House_mouse_PDP.jpeg,sortOrder:5}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325438

Product Specific Information

MA1-750 detects prion protein (PrP) protein from a variety of species that have the conserved n-IHFG-c epitope, including agriculturally important animal species such as sheep, bovine, deer, and elk. MA1-750 has been successfully used in Western blot, immunohistochemistry and ELISA procedures. By Western blot, this antibody detects a 33-35 kDa protein from normal animals and a 27-30 kDa protein which represents PrP in brain protease treated tissue extracts from infected animals. Immunohistochemical staining of PrP(Sc) in infected sheep brain with MA1-750 results in intense staining of the spongiform lesions. In immunohistochemical procedures this antibody detects only the PrP(Sc) and not PrP(C). The MA1-750 immunogen is a synthetic peptide corresponding to residues S(146) R P L I H F G S D Y E D R(159) of bovine PrP. This peptide (Cat. # PEP-051) is available for use in neutralization and control experiments. MA1-750 is known to specifically recognize a conserved epitope of the PrP(Sc) protein comprising the amino acids n-IHFG-c.

Target Information

Human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.