Thermo Fisher Scientific ITFG2 Monoclonal Antibody (OTI 3B3), TrueMAB
ITFG2 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, ICC/IF, Flow Cytometry에 사용 가능. Human 시료 반응성. Affinity chromatography로 정제된 액상 형태이며, PBS/BSA/glycerol buffer에 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI 3B3 |
| Immunogen | Full length human recombinant protein of human ITFG2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.33 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
As part of the KICSTOR complex, ITFG2 functions in the amino acid-sensing branch of the TORC1 signaling pathway. It recruits the GATOR1 complex to lysosomal membranes in an amino acid-independent manner, enabling its interaction with GATOR2 and RAG GTPases. ITFG2 acts upstream of the RAG GTPases and is required to negatively regulate mTORC1 signaling in the absence of amino acids. Without the KICSTOR complex, mTORC1 remains constitutively localized to the lysosome and activated. The KICSTOR complex is also likely involved in the regulation of mTORC1 by glucose.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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