Thermo Fisher Scientific AKT2 Monoclonal Antibody (11F6.B2), Biotin
Human AKT2 단백질을 인식하는 Biotin 결합 Rat 단일클론 항체. Western blot, IHC, ELISA 등 다양한 응용 가능. 인산화 및 비인산화 형태 모두 검출. Protein G 정제, 장기보관 시 -20°C 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 11F6.B2 |
| Immunogen | Synthetic peptide corresponding to internal residues of human AKT2 protein |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.5M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
Store vial at 4°C prior to restoration. Restore with deionized water (or equivalent). For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Cross-reactivity with AKT2 from other species has not been determined; however, the sequence of the immunogen shows 88% identity to mouse and 90% identity with rat, therefore cross-reactivity is expected.
Target Information
AKT2 is a phosphoinositide-dependent serine/threonine protein kinase enriched in insulin-responsive tissues and implicated in the metabolic actions of the hormone. Akt is involved in many cellular signaling pathways and acts as a transducer of functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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