Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), eBioscience
Mouse CD45 단백질을 인식하는 Rat IgG2b 단클론 항체로, Flow Cytometry, IHC, IP 등 다양한 응용 가능. Unconjugated 형이며 0.5 mg/mL 농도, PBS buffer에 sodium azide 포함. 마우스 혈액세포 분석 및 면역학 연구에 적합.
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Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 |
| Immunohistochemistry (IHC) | Assay-Dependent | 64 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 7 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 14 |
| Immunocytochemistry (ICC/IF) | - | 16 |
| Flow Cytometry (Flow) | 0.5 µg/test | 339 |
| ELISA | - | 1 |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Functional Assay | Assay-Dependent | 3 |
| In vitro Assay (IV) | - | 1 |
| Miscellaneous PubMed | - | 1 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 30-F11 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467250 |
Available Conjugate Formats
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780, Biotin
- Brilliant UV, Brilliant Violet 시리즈
- eFluor, FITC, PE, PE-Cyanine, PerCP-Cyanine
- Super Bright 시리즈
- NovaFluor Blue, Red, Violet, Yellow 시리즈
- Functional Grade
- Custom conjugation 요청 가능
Product Specific Information
The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.
Applications Reported:
Flow cytometric analysis, immunoprecipitation, immunohistochemical staining, complement-dependent cytotoxicity. (Functional Grade purified 30-F11 is recommended for functional assays.)
Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. Recommended ≤0.5 µg per test (100 µL final volume). Cell number may vary (10⁵–10⁸ cells/test). Optimize titration for best performance.
Purity: >90% by SDS-PAGE
Aggregation: <10% by HPLC
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells. It constitutes ~10% of lymphocyte surface proteins and is absent in non-hematopoietic cells. CD45 regulates Src-family kinases, antigen receptor signaling, and cytokine receptor pathways. Isoforms arise from alternative splicing and differ in extracellular domains. CD45 plays key roles in lymphocyte activation, survival, and immune regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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