Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), eBioscience
마우스 CD45.2 단백질을 인식하는 단클론 항체로, Flow Cytometry, IHC(F), IP 등에 사용 가능. 다양한 형광 및 효소 표지 형식 제공. 고순도(>90%) 및 낮은 응집도(<10%) 확보. 연구용으로 면역세포 분석 및 신호전달 연구에 적합.
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Thermo Fisher Scientific CD45.2 Monoclonal Antibody (Clone 104)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 5 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Flow Cytometry (Flow) | 0.5 µg/test | View 122 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Functional Assay | - | View 1 publication |
| Miscellaneous (PubMed) | - | View 2 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 104 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467261 |
Available Conjugate Formats
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV Series (395–805)
- Brilliant Violet Series (421–786)
- eFluor 450, eFluor 506
- FITC
- NovaFluor Blue Series (510–660)
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright Series (436–780)
- Request custom conjugation
Product Specific Information
The 104 monoclonal antibody reacts with the mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.2-expressing mouse strains such as C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed by all leukocytes in these strains.
Applications Reported:
Flow cytometric analysis, immunoprecipitation, immunohistochemical staining of frozen tissue sections.
(Biotinylated 104 is recommended for IHC, Product #13-0454)
Applications Tested:
Validated by flow cytometric analysis of mouse splenocytes at ≤0.5 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Optimal titration recommended.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD45.2 (leukocyte common antigen, LCA) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. It plays a key role in lymphocyte development and antigen signaling by regulating Src-family kinases.
CD45.2 exists in multiple isoforms due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic domains. Isoform expression varies by cell type and physiological state. In addition to immunoreceptor signaling, CD45.2 promotes cell survival via integrin-mediated pathways and contributes to DNA fragmentation during apoptosis. CD45RA is an isoform with restricted expression across lymphoid subtypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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