Thermo Fisher Scientific CaMKII alpha Monoclonal Antibody (6G9), FITC
CaMKII alpha 단백질을 인식하는 FITC 결합 단일클론 항체로, WB, IHC, ICC, ELISA 등 다양한 응용에 적합. Bovine, Human, Mouse, Rat 반응성. 단백질 G 정제, 보존제 없음, 4°C 암소 보관. 형광 분석용으로 최적화된 고감도 항체.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:10,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:2,000–1:10,000 | |
| Immunocytochemistry (ICC/IF) | 1:50 | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent | |
| Radioimmune Assays (RIA) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6G9 |
| Immunogen | Partially purified rat CaMKII |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS (pH 7.2), with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931598 |
Additional Formats
- APC (Cat. No. MA5-45143)
- PerCP (Cat. No. MA5-45145)
- Custom conjugation available upon request
Product Specific Information
- 0.1 µg/mL was sufficient for detection of CaMKII in 20 µg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary antibody.
- Detects approximately 50–60 kDa.
- Recognizes both phosphorylated and non-phosphorylated forms.
Target Information
CAMK2A (CaMKII alpha) is a serine/threonine kinase that regulates various cellular processes including:
- Neuronal signaling and synaptic plasticity
- Gene expression and carbohydrate metabolism
- Cytoskeletal organization and calcium homeostasis
Autophosphorylation at threonine 286 enables CAMK2A to switch from Ca2+/calmodulin-dependent to independent activity. It plays a key role in dendritic spine formation, synaptic remodeling, and regulation of Ca2+ transport in muscle cells. In neurons, CAMK2A contributes to actin cytoskeleton reorganization during plasticity by binding and bundling actin filaments independently of its kinase activity.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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