Thermo Fisher Scientific CD33 Monoclonal Antibody (9A11-CD33), APC, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: View 1 publication

Product Specifications

Additional Available Formats

• Alexa Fluor 647
• PE
• PE-Cyanine7
• Super Bright 436
• Super Bright 702
• Request custom conjugation

Product Specific Information

This monoclonal antibody (clone 9A11-CD33) reacts with mouse CD33, also known as Siglec3 or gp67.
CD33 belongs to the sialic acid-binding immunoglobulin lectin (Siglec) family, binding sialic acid-containing glycans on cell surface glycoproteins and glycolipids. Mouse CD33 binds short O-linked glycans of certain mucins and sialyl-Tn epitopes. It is expressed by mature granulocytes, immature myeloid cells, and microglia. Unlike human CD33, mouse CD33 lacks a cytoplasmic ITIM domain and exists as two splice variants with different cytoplasmic tails. In microglia, CD33 inhibits phagocytosis of toxic amyloid beta.

Clone 9A11 has been used to characterize mouse CD33 surface expression and function.
View Article

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes; ≤0.5 µg/test (100 µL final volume).
Cell number: 10⁵–10⁸ cells/test. Optimize titration for best results.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD33 is a transmembrane protein of the Siglec family, containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that recruit SHP-1 and SHP-2 phosphatases. These motifs also mediate receptor removal via ubiquitin pathways. CD33 is expressed on myelomonocytic lineage cells, binds sialic acid residues in N- and O-glycans, and serves as a therapeutic target in acute myeloid leukemia. It is present on granulocyte and macrophage precursors in bone marrow but absent from pluripotent stem cells. CD33 is also expressed on peripheral monocytes and helps distinguish myelogenous leukemia cells from lymphoid or erythroid leukemias.

Diseases associated with CD33 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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