Thermo Fisher Scientific Cathepsin L Monoclonal Antibody (204101)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

5-25 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

5-25 µg/mL

-

Immunoprecipitation (IP)

25 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

204101

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cathepsin L Glu113-Val333 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cathepsin L,uniProtId:P07711-1,ncbiNodeId:9606,antigenRange:113-333,antigenLength:333,antigenImageFileName:MA5-23891_Cathepsin_L_P07711-1_Rat.svg,antigenImageFileNamePDP:MA5-23891_Cathepsin_L_P07711-1_Rat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609641

Product Specific Information

In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cathepsin B, C, L2, O, S, or X/Z/P is observed. In Western blots, both the pro and active forms of recombinant human and mouse Cathspsin L are recognized.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.