Thermo Fisher Scientific Sclerostin Monoclonal Antibody (220902)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

ELISA (ELISA)

0.5-2 µg/mL (Detection), 2-8 µg/mL (Capture)

-

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

220902

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SOST/Sclerostin Gln24-Tyr213 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Sclerostin,uniProtId:Q9BQB4-1,ncbiNodeId:9606,antigenRange:24-213,antigenLength:213,antigenImageFileName:MA5-23897_Sclerostin_Q9BQB4-1_House_mouse.svg,antigenImageFileNamePDP:MA5-23897_Sclerostin_Q9BQB4-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2605787

Product Specific Information

In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse SOST is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

Sclerostin, a glycoprotein predominantly secreted by osteocytes, is a member of the Cerberus/DAN family of putative BMP antagonists that functions as an endogenous regulator of the canonical Wnt signaling pathway and an inhibitory regulator of bone homeostasis. Although expressed nearly exclusively by osteocytes, sclerostin can also be found at significant levels elsewhere, such as bone, bone marrow, cartilage, the kidney, and the liver, and has also been shown to be produced by hypertrophic chondrocytes and cementocytes. Like DKK family members DKK-1 and DKK-4, sclerostin plays an important regulatory role in the Wnt/beta-catenin signaling pathway by forming inhibitory complexes with LDL Receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/beta-catenin signaling system. LRP5 and LRP6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/beta-catenin signaling cascade. Sclerostin has also been shown to interact directly with LRP4 via its extracellular domain to facilitate inhibition of Wnt signaling, and can catabolically promote osteoclast activity by increasing osteocyte expression of RANKL. Sclerostin`s critical involvement in the regulation of bone formation and resorption is emphasized by two bone dysplasia disorders, sclerosteosis and van Buchem disease (VBD), caused by rare autosomal recessive mutations that result in progressive bone overgrowth and hypermineralization due to markedly decreased sclerostin levels.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.