Thermo Fisher Scientific CD68 Monoclonal Antibody (OTI8A4), TrueMAB
CD68 단백질을 인식하는 Mouse monoclonal antibody로, Western blot 및 IHC(Paraffin) 검증 완료. Human 시료에 반응하며, Lyophilized 형태로 제공. 고순도 친화 크로마토그래피 정제 및 트레할로오스 기반 안정화 완충액 포함.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI8A4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 22–319 of human CD68 produced in SF9 cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on intracellular lysosomes of monocytes and macrophages, and to a lesser extent by dendritic cells and peripheral blood granulocytes.
It plays roles in phagocytic activities of tissue macrophages, including intracellular lysosomal metabolism and extracellular cell–cell and cell–pathogen interactions.
CD68 expression is observed in interdigitating reticulum cells in tonsil and certain histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma.
Elevated CD68 expression has been demonstrated on CD34+ cells in various human malignancies, including several AML studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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