Thermo Fisher Scientific HNF4A Monoclonal Antibody (H1415)
HNF4A 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, WB, IHC, ICC, ELISA 등 다양한 응용에 적합합니다. 인간, 마우스, 랫에서 교차 반응하며, 냉장 또는 냉동 보관이 가능합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 2 publications |
| Immunohistochemistry (IHC) | 10–20 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100 | View 1 publication |
| ELISA | 0.3 µg/mL | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
| ChIP assay (ChIP) | Assay-dependent | - |
| Gel Shift (GS) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Mouse, Non-human primate |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | H1415 |
| Immunogen | Baculovirus-expressed recombinant human HNF4-alpha (393–461 aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | Maintain refrigerated at 2–8°C for up to 1 month. For long-term storage, store at -20°C. |
| Shipping conditions | Wet ice |
| RRID | AB_2532197 |
Product Specific Information
This antibody specifically recognizes human HNF4 alpha isoforms 1, 2, 4, 5, 7, and 8, and cross-reacts with mouse and rat HNF4 alpha isoforms 1, 2, 4, 5, 7, and 8.
Target Information
Hepatocyte nuclear factor 4 alpha (HNF4α) is an orphan nuclear receptor expressed in the liver, kidney, intestine, and pancreas. Mutations in HNF4α are associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds DNA as an exclusive homodimer. The HNF4α gene is alternatively spliced and may generate up to nine isoforms (HNF4α1–HNF4α9).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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