Thermo Fisher Scientific ZEB1 Monoclonal Antibody (OTI7E12), TrueMAB
ZEB1 단백질을 표적으로 하는 Mouse IgG2a 단일클론 항체로, WB 및 IHC(P)에서 검증됨. 인간 시료에 반응하며, carrier-free 형태로 제공되어 결합 실험에 적합. -20°C 보관, 재구성 후 1 mg/mL 농도로 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7E12 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–350 of human ZEB1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
ZEB1 (Zinc finger E-box-binding homeobox 1) is a transcriptional repressor that inhibits IL-2 gene expression and modulates ATP1A1 promoter activity depending on cell type and cDNA quantity. It represses E-cadherin promoters, inducing epithelial-mesenchymal transition (EMT) via SMARCA4/BRG1 recruitment. ZEB1 also represses BCL6 transcription with CTBP1 and regulates neuronal differentiation by repressing RCOR1 activation. Mutations in ZEB1 are associated with corneal dystrophy, posterior polymorphous corneal dystrophy, and Fuchs endothelial dystrophy.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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