Thermo Fisher Scientific Zap-70 Recombinant Rabbit Monoclonal Antibody (JU08-39)
ZAP-70 단백질을 인식하는 고특이성 재조합 토끼 모노클론 항체. Western blot, IHC, ICC, Flow cytometry, IP 등 다양한 응용 가능. HEK293 세포 발현, Protein A 정제, 높은 재현성과 감도 제공. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
| Immunoprecipitation (IP) | 1:10–1:50 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JU08-39 |
| Immunogen | Synthetic peptide within Human ZAP70 aa 570–619 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810100 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal production candidates.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase and a member of the Syk family. It is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation.
Upon TCR engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319 within the interdomain B (between SH2 and kinase domains).
- Tyrosine 315 (Vav binding site) and 319 (Lck binding site) enhance ZAP70 function in lymphocyte signaling.
- Tyrosine 292 terminates transient activation and attenuates signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: MA5-32824_Zap-70_P43403-1_Rabbit.svg, MA5-32824_Zap-70_P43403-1_Rabbit_PDP.jpeg)
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