Thermo Fisher Scientific ATM Polyclonal Antibody, DyLight 550
ATM 단백질을 표적하는 DyLight 550 형광 표지된 Rabbit Polyclonal Antibody로 다양한 종에 반응합니다. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 연구 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공합니다.
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Thermo Fisher Scientific ATM Polyclonal Antibody, DyLight 550
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Dog, Human, Marsupial, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A fragment of human ATM protein expressed in E. coli, corresponding to the C-terminal region (within the last third of the protein sequence). |
| Conjugate | DyLight™ 550 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Liquid |
| Concentration | 1.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11151978 |
Target Information
Ataxia-telangiectasia Mutated (ATM) is a protein belonging to the PI3/PI4 kinase family. The ATM gene encodes a kinase that phosphorylates proteins involved in cell-cycle regulation, including P53, Mdm2, BRCA1, and SMC1. ATM plays a crucial role in repairing double-stranded DNA breaks caused by ionizing radiation and other mutagens. Its C-terminal region shows homology to catalytic domains of phosphatidylinositol 3-kinases. Upon DNA damage, ATM becomes autophosphorylated and activates downstream kinases regulating cell cycle, apoptosis, and DNA repair. Mutations in ATM are associated with ataxia-telangiectasia, a disorder characterized by neurodegeneration, immunodeficiency, and cancer susceptibility.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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