Thermo Fisher Scientific ATG3 Recombinant Rabbit Monoclonal Antibody (JU00-35)
ATG3 단백질을 표적으로 하는 재조합 토끼 단클론 항체. Western blot과 IHC(P)에서 검증됨. 높은 특이성과 재현성을 제공하며, 단백질 A로 정제된 액상 형태. 자가포식 관련 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JU00-35 |
| Immunogen | Synthetic peptide within Human Apg3 (amino acids 1–50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810091 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
Target Information
ATG3 (APG3) is a conjugating enzyme essential for APG8 lipidation, a key step in autophagy initiation.
Autophagy is a cellular degradation process important for growth control and nutrient recycling.
ATG3 acts as an E2-like enzyme during the early stages of autophagosome formation by catalyzing the formation of the Atg8–phosphatidylethanolamine (Atg8–PE) conjugate, which is critical for autophagy.
This process is negatively regulated by TOR (Target of Rapamycin) through phosphorylation of autophagy protein APG1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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