Thermo Fisher Scientific MMP2 Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific MMP2 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MYNFFPRKPK WDKNQITYRI IGYTPDLDPE TVDDAFARAF QVWSDVTPLR FSRIHDGEAD IMINFGRWEH GDGYPFDGKD GLLAHAFAPG TGVGGDSHFD DDELWTLGEG QVVRVKYGNA DGEYCKFPFL FNGKEYNSCT DTGRSDGFLW CSTTYNFEKD GKYGFCPHEA LFTMGGNAEG QPCKFPFRFQ GTSYDSCTTE GRTDGYRWCG TTEDYDRDKK YGFCPETAMS TVGGNSEGAP CVFPFTFLGN KYESCTSAGR SDGKMWCATT ANYDDDRKWG FCPDQGYSLF LVAAHEFGHA MGLEHSQDPG ALMAPIYTYT KNFRLSQDDI KGIQELYGAS PDIDLGTGPT PTLGPVTPEI CKQDIVFDGI AQIRGEIFFF KDRFIWRTVT PRDKPMGPLL VATFWPELPE KIDAVYEAPQ EEKAVFFAGN EYWIYSASTL ERGYPKPLTS LGLPPDVQRV DAAFNWSKNK KTYIFAGDKF WRYNEVKKKM DPGFPKLIAD AWNAIPDNLD AVVDLQGGGH SYFFKGAYYL KLENQSLKSV KFGSIKSDWL GC

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MMP-2. Anti-Human MMP-2-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human MMP-2 by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MMP-2 (500-P307) as a capture antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human MMP-2.

Western Blot:
To detect Human MMP-2 by Western Blot, use at 0.1–0.2 µg/mL. When used with compatible development reagents, the detection limit for Recombinant Human MMP-2 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P307BT-1MG will be provided as 2 × 500 µg.

Target Information

Matrix metalloproteinases (MMPs) are proteolytic enzymes capable of degrading connective tissue components. MMPs share a common activation mechanism, a conserved amino acid sequence in the metal-binding active site, and are inhibited by tissue inhibitors of metalloproteinases (TIMPs).
MMP2 is synthesized as a 631 amino acid proenzyme activated by cleavage of the first 80 amino acids. It includes propeptide, catalytic, and hemopexin domains.
MMP2 plays a key role in tissue remodeling and angiogenesis. Mutations in the MMP-2 gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms of MMP-2 have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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