Thermo Fisher Scientific MIP-3 alpha Polyclonal Antibody, HRP
MIP-3 alpha(또는 CCL20)을 인식하는 HRP 결합 폴리클로날 항체로, Western blot, IHC, ELISA 등 다양한 면역분석에 사용됩니다. 마우스 및 랫트 시료에 반응하며, 안정적인 HRP 표지와 높은 특이성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,500 |
| ELISA | 1:10,000–1:50,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MIP-3 alpha Antibody Peroxidase Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse MIP-3alpha protein. |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Storage Buffer | 0.02M potassium phosphate/whole serum, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Product is stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
- In ELISA and other immunoreactive assays, this antibody recognizes both native and recombinant mouse MIP-3 alpha in cell supernatants and certain body fluids.
- A control of similarly diluted normal rabbit IgG is recommended.
Target Information
Macrophage Inflammatory Protein-3 alpha (MIP-3alpha), also known as CCL20, is expressed in the liver, lungs, lymph nodes, and peripheral blood lymphocytes. It is upregulated by inflammatory signals and downregulated by the anti-inflammatory cytokine IL-10. MIP-3alpha signals through the G protein-coupled receptor CCR6 and acts as a chemoattractant to lymphocytes and dendritic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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