Thermo Fisher Scientific SRD5A1 Polyclonal Antibody

Applications

• Western Blot (WB): 1:500–1:1,000

Product Specifications

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is greater than 95% as determined by SDS-PAGE.

Target Information

Steroid 5alpha-Reductase is an essential enzyme in androgen physiology, catalyzing the conversion of testosterone into the more potent 5alpha-dihydrotestosterone (DHT), which mediates androgen effects on target tissues. The enzyme exists as two isoforms:

• Type 1: Expressed mainly in the skin.
• Type 2: Expressed mainly in the prostate.

In cultured human skin cells, 5alpha-Reductase 1 shows protein heterogeneity and variable transcriptional and translational expression. It is expressed throughout the hair follicle, while 5alpha-Reductase 2 is limited to mesenchymal portions. 5alpha-Reductase 1 is also expressed in human breast carcinoma and may play a role in local androgen metabolism and inhibition of cancer cell proliferation in hormone-dependent breast carcinoma. Furthermore, the 5alpha-Reductase–3alpha-hydroxysteroid dehydrogenase complex is present in the human brain, suggesting involvement in neurosteroid synthesis or catabolism of neurotoxic steroids.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.