Thermo Fisher Scientific B7-H3 Monoclonal Antibody (6A1)
인간 CD276(B7-H3)을 인식하는 Mouse IgG1 단일클론 항체로, WB, IHC, Flow, ELISA에 사용 가능. 비접합형 액상 형태이며, 4°C 단기 보관 및 -20°C 장기 보관 권장. 연구용으로 면역반응 및 암 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
| Miscellaneous PubMed (Misc) | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6A1 |
| Immunogen | Purified recombinant fragment of human CD276 (A.A. 30–130) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10987360 |
Product Specific Information
MA5-15693 targets CD276 in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human CD276 expressed in E. coli.
MA5-15693 detects CD276 with a predicted molecular weight of approximately 57 kDa.
Target Information
CD276, also known as B7-H3, is a member of the B7 family of co-stimulatory molecules.
It is a type I transmembrane protein that induces proliferation of CD4⁺ and CD8⁺ T cells, enhances cytotoxic T cell generation, and selectively stimulates interferon gamma production.
Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines and is also found in peripheral tissues such as the heart, kidney, testis, and colon.
In humans, CD276 exists as two isoforms resulting from gene duplication and differential splicing.
CD276 has therapeutic potential in regulating cell-mediated immune responses to cancer, particularly in combination with anti-angiogenic therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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