Thermo Fisher Scientific SEMA4D Polyclonal Antibody
Human, Mouse, Rat 반응성의 Rabbit Polyclonal 항체로, Western blot, IHC, Flow cytometry, ELISA 등에 사용 가능. KLH 결합 합성 펩타이드(aa 75-170)로 면역화. Protein A 정제, 1 mg/mL 농도, TBS/glycerol/BSA buffer에 보관. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human SEMA4D, amino acids 75–170 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Semaphorins are neuronal chemorepellants that guide pioneering neurons during nervous system development.
CD100 (also known as semaphorin 4D) is a 150 kDa homodimer that induces B cell aggregation and enhances viability in vitro.
It modulates CD40–CD40L B cell signaling, promoting aggregation and survival while downregulating CD23 expression.
CD72 acts as a lymphocyte receptor for CD100, regulating B cell signaling.
CD100 is expressed on resting and PHA-stimulated T cells, weakly on NK cells, EBV-transformed B cells, monocytes, and tumor/peripheral blood T cell lines, and at higher density on activated T and B cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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