Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), NovaFluor Blue 585, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.8 µg)/test

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, eFluor 450, FITC, Functional Grade, NovaFluor Blue 510, NovaFluor Blue 555, NovaFluor Blue 610-70S, NovaFluor Blue 660-120S, NovaFluor Blue 690, NovaFluor Blue 725, NovaFluor Blue 760, NovaFluor Blue 800, NovaFluor Red 700, NovaFluor Red 710, NovaFluor Red 725, NovaFluor Red 755, NovaFluor UV 765, NovaFluor Violet 690, NovaFluor Violet 800, NovaFluor Yellow 590, NovaFluor Yellow 610, NovaFluor Yellow 660, NovaFluor Yellow 690, NovaFluor Yellow 700, NovaFluor Yellow 730, NovaFluor Yellow 810, PE, PE-Cyanine5, PE-Cyanine7, PerCP-eFluor 710, Super Bright 436, Super Bright 600, Super Bright 645, Super Bright 702, Super Bright 780.

Product Specific Information

The OKT8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as a heterodimer (CD8αβ) or homodimer (CD8αα). CD8a is found on most thymocytes and subsets of mature T and NK cells. It binds MHC class I and associates with p56lck, influencing T-cell development and activation.

Preliminary testing shows OKT8, RPA-T8, and HIT8a clones do not compete for binding to human peripheral blood leukocytes, suggesting distinct epitopes.

Each product includes 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and validated for flow cytometry (5 µL (0.8 µg)/test)
  • A test = antibody amount for 100 µL staining volume
  • Cell range: 10⁵–10⁸ cells/test

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• Use CellBlox Plus Blocking Buffer (Product # C001T06F01) for optimal staining.
  • Add 5 µL per sample before mixing with cells
  • For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells)

NovaFluor conjugates utilize Phiton™ technology, providing engineered fluorescent signatures optimized for minimal spillover.

• Excitation: 494 nm
• Emission: 585 nm
• Laser: 488 nm (Blue)

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family, essential for T-cell receptor (TCR) co-receptor function with MHC class I molecules. It is primarily expressed on cytotoxic T cells, NK cells, monocytes, and dendritic cells, existing as CD8αβ heterodimers or CD8αα homodimers. CD8 engagement triggers Lck-mediated signaling, leading to lymphokine production and cytotoxic T lymphocyte (CTL) activation, critical for pathogen and tumor clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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