Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), NovaFluor Blue 610-30S, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, eFluor 450, FITC, Functional Grade, NovaFluor Blue 510, NovaFluor Blue 555, NovaFluor Blue 585, NovaFluor Blue 610-70S, NovaFluor Blue 660-120S, NovaFluor Blue 690, NovaFluor Blue 725, NovaFluor Blue 760, NovaFluor Blue 800, NovaFluor Red 700, NovaFluor Red 710, NovaFluor Red 725, NovaFluor Red 755, NovaFluor UV 765, NovaFluor Violet 690, NovaFluor Violet 800, NovaFluor Yellow 590, NovaFluor Yellow 610, NovaFluor Yellow 660, NovaFluor Yellow 690, NovaFluor Yellow 700, NovaFluor Yellow 730, NovaFluor Yellow 810, PE, PE-Cyanine5, PE-Cyanine7, PerCP-eFluor 710, Super Bright 436, Super Bright 600, Super Bright 645, Super Bright 702, Super Bright 780.
Custom conjugation available upon request.

Product Specific Information

The OKT8 monoclonal antibody reacts with the human CD8a molecule (32–34 kDa), expressed as CD8αβ heterodimer or CD8αα homodimer. It is found on most thymocytes, a subset of mature T cells, and NK cells. CD8 binds to MHC class I and associates with protein tyrosine kinase p56lck, playing a role in T-cell development and activation.

OKT8, RPA-T8, and HIT8a clones do not compete for binding to human peripheral blood leukocytes, suggesting distinct epitope recognition.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells.
Recommended use: 5 µL (0.5 µg)/test in 100 µL final volume (10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• CellBlox Plus Blocking Buffer (Product # C001T06F01) must be used for optimal staining.
  • Add 5 µL per sample to antibody cocktails before combining with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, based on novel nucleic acid dye structures engineered for optimized fluorescence with minimal spillover and spread.

Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family. It functions as a co-receptor with TCR for MHC class I molecules, facilitating T cell activation and differentiation.
CD8 is expressed on cytotoxic T lymphocytes, NK cells, monocytes, and dendritic cells as either CD8αβ heterodimers or CD8αα homodimers.
Upon MHC-I/peptide complex binding, CD8 recruits Lck kinase, leading to activation of cytotoxic T lymphocytes (CTLs) involved in pathogen and tumor cell clearance.
Differentiation of naïve CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지