Thermo Fisher Scientific USP5 Monoclonal Antibody (OTI2E3), TrueMAB
USP5 단백질을 인식하는 Mouse IgG2a 단일클론 항체로 Western Blot 및 IHC(Paraffin)에 사용 가능. 인간, 개, 쥐 반응성. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2E3 |
| Immunogen | Full length human recombinant protein of human USP5 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
USP5 (ubiquitin carboxyl-terminal hydrolase 5) cleaves linear and branched multiubiquitin polymers, with a preference for branched forms.
It participates in disassembly of unanchored Lys-48-linked polyubiquitin and binds linear and Lys-63 linked polyubiquitin with lower affinity.
Knock-down of USP5 results in accumulation of p53/TP53 and increased transcriptional activity, as unanchored polyubiquitin competes with ubiquitinated p53/TP53 but not MDM2 for proteasomal recognition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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