Thermo Fisher Scientific Phospho-ATF2 (Thr52) Polyclonal Antibody
Phospho-ATF2 (Thr52) 특이적 항체로 Western blot에 적합. 인간 및 생쥐에 반응하며, 다양한 종에서도 예측 반응. Affinity chromatography로 정제된 rabbit polyclonal IgG. -20°C에서 보관, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr52 of human ATF2 conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 0.1mg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2552701 |
Product Specific Information
This antibody is predicted to react with bovine, chicken, mouse, non-human primate, rat, and Xenopus based on 100% sequence homology.
Target Information
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins.
ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways.
Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2.
Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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