Thermo Fisher Scientific ZNF281 Polyclonal Antibody
Thermo Fisher Scientific의 ZNF281 Polyclonal Antibody는 인간 및 생쥐 시료에 반응하며, Western blot과 Immunocytochemistry에 적합합니다. 항원 친화 크로마토그래피로 정제된 액상 형태로 제공되며, 장기 보관 시 -20°C에서 안정적입니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 19 amino acid peptide near the carboxy terminus of human ZFP281 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153148 |
Product Specific Information
A suggested positive control is A-20 cell lysate.
PA5-21121 can be used with blocking peptide PEP-1235.
Target Information
ZFP281, also known as ZBP-99, is a member of a conserved family of transcription factors that interact with GC-rich promoters. It contains four Kruppel-like zinc fingers and is highly homologous to ZBP-89, another regulator of ornithine decarboxylase gene expression.
ZFP281 interacts with various transcription factors such as Sp1 to repress vimentin gene transcription. It also regulates embryonic stem cell pluripotency by directly controlling specific target genes and physically interacts with transcription factors POU5F1, Sox2, and NANOG.
Gene expression microarray analyses indicate that ZFP281 can both activate and repress target genes upon knockdown, demonstrating its bifunctional role in stem cell gene regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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