Thermo Fisher Scientific CD41a Monoclonal Antibody (HIP8), NovaFluor Blue 510, eBioscience

Thermo Fisher Scientific CD41a Monoclonal Antibody (HIP8), NovaFluor Blue 510

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• NovaFluor Blue 585
• NovaFluor Blue 660-120S
• NovaFluor Yellow 590
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Product Specific Information

The HIP8 monoclonal antibody reacts with the human CD41 molecule (integrin αIIb, platelet GPIIb). CD41 associates with integrin β3 (CD61) and is expressed by megakaryocytes and platelets. The CD41/CD61 complex acts as a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin, and thrombospondin, mediating platelet aggregation. HIP8 blocks platelet aggregation.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Recommended usage: 5 µL (0.6 µg) per test in 100 µL final volume. Suitable for 10⁵–10⁸ cells/test.

Note:
NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):
Required for optimal staining with NovaFluor conjugates. Add 5 µL per sample to antibody cocktails prior to mixing with cells. For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology based on nucleic acid dye structures engineered for optimized fluorescence with minimal spillover and spread.

Excitation: 496 nm
Emission: 511 nm
Laser: 488 nm (Blue)

Target Information

CD41 (platelet glycoprotein IIb, ITGA2B) comprises two subunits (α: 120 kDa, β: 23 kDa) that interact with CD61 in the presence of calcium to form a functional adhesive receptor. CD41 mediates platelet aggregation and binds proteins such as von Willebrand factor, fibrinogen, fibronectin, and vitronectin upon vascular injury. It is mainly expressed on megakaryocyte-platelet lineage and participates in early hematopoietic differentiation.
Associated diseases include Glanzmann Thrombasthenia and Platelet type-16 bleeding disorder.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

제품 이미지