Thermo Fisher Scientific Aurora B Monoclonal Antibody (AURKB, 1593)
인간 Aurora B 단백질을 인식하는 mouse monoclonal antibody로, IHC(P) 및 peptide array에 사용 가능. Recombinant fragment(aa 89-251)를 면역원으로 제작. Protein A/G 정제, 1 mg/mL 농도, PBS(pH 7.4) 용액 형태. 연구용으로만 사용.
- 카탈로그번호
- 9212-MSM3-P1ABX
- 판매단위
- pk
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Peptide Array (Array)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AURKB, 1593 |
| Immunogen | Recombinant fragment (around aa 89–251) of human Aurora B protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Aurora kinases associate with microtubules during chromosome movement and segregation.
AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to specialized microtubules called K-fibers.
This complex functions in chromosome alignment and separation, histone modification, and cytokinesis.
The Chromosomal Passenger Complex (CPC) includes three non-enzymatic subunits: inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localization, stability, and possibly substrate specificity of Aurora B.
Two CPCs exist during mitosis: one containing all four members and another consisting of INCENP and Aurora B.
The quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP–AurB complex may be responsible for modifying histone H3.
AurB is overexpressed in several tumors, including primary breast and colon tumor samples. Its expression appears linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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