Thermo Fisher Scientific Aurora B Monoclonal Antibody (AURKB, 1593)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.

Target Information

Aurora kinases associate with microtubules during chromosome movement and segregation.
AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers.
This complex functions in chromosome alignment and separation, histone modification, and cytokinesis.

The Chromosomal Passenger Complex (CPC) includes three non-enzymatic subunits — inner centromere protein (INCENP), survivin, and borealin — which determine the activity, localization, stability, and possibly even the substrate specificity of Aurora B.
Two CPCs exist during mitosis: one containing all four members and another consisting of INCENP and Aurora B.
The quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP–AurB complex may be responsible for modifying histone H3.

Aurora B is overexpressed in several tumors, including primary breast and colon tumor samples.
Its expression in tumors appears linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.