Thermo Fisher Scientific PELI1 Monoclonal Antibody (OTI1D9), TrueMAB
PELI1 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB 및 IHC(P) 실험에 사용 가능. Human 대상 반응성. Lyophilized 형태로 제공되며, PBS 버퍼에 trehalose 포함. 연구용으로 NF-κB 신호전달 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1D9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–245 of human PELI1 produced in E.coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The Pellino proteins are a family of E3 ubiquitin ligases that act as upstream mediators in Toll-like receptor (TLR) pathways, leading to MAP kinase and transcription factor activation.
Pellino 1 interacts with the IRAK4–IRAK–TRAF6 complex to mediate interleukin-1 signaling and activate NF-κB. It catalyzes polyubiquitination of IRAK1 and is regulated via phosphorylation (by IRAK1, IRAK4, IKK-ε, or TBK1), ubiquitination, and sumoylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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