Thermo Fisher Scientific Alpha-1-Antitrypsin (SERPINA1) Monoclonal Antibody (AAT, 1379)
인간 Alpha-1-Antitrypsin(SERPINA1)을 인식하는 마우스 단클론 항체로 WB 및 IHC(P) 적용 가능. 단백질 A/G로 정제된 액상형 항체이며, 보존제가 포함되지 않음. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AAT, 1379 |
| Immunogen | Recombinant human Alpha-1-Antitrypsin (AAT) protein fragment |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.
Target Information
Alpha-1-Antitrypsin (Alpha-1-AT) is synthesized in the liver and functions as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung.
In the absence of alpha-1-AT, these enzymes are not inhibited and may digest pulmonary parenchyma.
Alpha-1-AT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn.
Increase in alpha-1-AT occurs as an acute phase response to tissue necrosis and inflammation.
Serum levels of alpha-1-AT are elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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