Thermo Fisher Scientific Alpha-1-Antitrypsin (SERPINA1) Monoclonal Antibody (AAT, 1379)
인간 Alpha-1-Antitrypsin(SERPINA1)을 인식하는 Mouse IgG1 단일클론 항체. Western blot 및 파라핀 절편 IHC에 사용 가능. 단백질 A/G로 정제된 액상 형태로 PBS 버퍼에 0.05% BSA와 sodium azide 함유. 연구용 전용 제품.
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Applications
Western Blot (WB)
- Tested Dilution: 1–2 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AAT, 1379 |
| Immunogen | Recombinant human Alpha-1-Antitrypsin (AAT) protein fragment (amino acids 41–179) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature (RT).
- For formalin-fixed tissues, heat tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, then cool at RT for 20 min.
Target Information
Alpha-1-Antitrypsin (Alpha-1-AT) is synthesized in the liver and acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin released during inflammatory reactions in the lung.
Deficiency of Alpha-1-AT is associated with chronic obstructive lung disease (emphysema) and, less frequently, hepatic cirrhosis in infants and respiratory distress of the newborn.
Increased Alpha-1-AT levels occur as an acute-phase response to tissue necrosis and inflammation. Serum levels are elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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