Thermo Fisher Scientific DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17), Alexa Fluor Plus 647

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Alexa Fluor Plus 594
• Biotin
• HRP
• Custom conjugation available

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal clones.
Advantages include:

• 높은 특이성과 민감도
• 로트 간 일관성
• 다양한 타겟에 대한 광범위한 면역 반응성

Alexa Fluor™ Plus antibodies provide brighter signals and improved signal-to-noise ratios compared to conventional Alexa Fluor conjugates, enabling superior imaging quality. The proprietary dye chemistry ensures enhanced fluorescence performance.

Usage Note:
Before use, briefly centrifuge the conjugate solution and use the supernatant only to remove possible aggregates. Optimal antibody dilution should be determined empirically depending on the application.

Tested Applications:
Validated for WB, ICC/IF, and Flow Cytometry using DYKDDDDK-tagged proteins.

Optical Properties:

• Excitation: 658 nm
• Emission: 675 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

DYKDDDDK tag (FLAG tag) is an eight–amino acid peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) widely used for protein purification, detection, and localization.
It can be fused to either terminus of a target protein, allowing easy identification and isolation using anti-FLAG antibodies.
The FLAG tag is hydrophilic, minimizing interference with protein folding or activity, and is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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