Thermo Fisher Scientific Calsequestrin Recombinant Rabbit Monoclonal Antibody (ARC2209)
Calsequestrin 단백질을 인식하는 토끼 단클론 항체로, Western blot, ICC/IF, ELISA에 적합합니다. HEK293 세포에서 발현된 재조합 항체이며, 고순도의 Affinity chromatography로 정제되었습니다. 심근 조직 연구 및 칼슘 결합 단백질 분석에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC2209 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 100–200 of human Calsequestrin 1 (NP_0012223) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.3 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897856 |
Product Specific Information
- Positive Samples: Rat heart
- Immunogen sequence:
EDKGVGFGLV DSEKDAAVAK KLGLTEVDSM YVFKGDEVIE YDGEFSADTI VEFLLDVLED PVELIEGERE LQAFENIEDE IKLIGYFKSK DSEHYKAFED A
Target Information
The sarcoplasmic reticulum (SR) is responsible for maintaining intracellular calcium levels in cardiac and skeletal muscle by storing and releasing calcium. Among several SR calcium-binding proteins, calsequestrin is the most prominent. It binds calcium ions within the SR during muscle relaxation and is localized to the junctional and corbular regions of the SR. Calsequestrin functions to localize calcium near the terminal cisternae, facilitating release into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity with moderate to low affinity for calcium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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