Thermo Fisher Scientific Calsequestrin Recombinant Rabbit Monoclonal Antibody (ARC2209)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC2209

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human Calsequestrin 1 (NP_0012223) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Calsequestrin,uniProtId:P31415-1,ncbiNodeId:9606,antigenRange:100-200,antigenLength:396,antigenImageFileName:MA5-37936_Calsequestrin_P31415-1_Rabbit.svg,antigenImageFileNamePDP:MA5-37936_Calsequestrin_P31415-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.3 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897856

Product Specific Information

Positive Samples: Rat heart

Immunogen sequence: EDKGVGFGLV DSEKDAAVAK KLGLTEVDSM YVFKGDEVIE YDGEFSADTI VEFLLDVLED PVELIEGERE LQAFENIEDE IKLIGYFKSK DSEHYKAFED A

Target Information

The sarcoplasmic reticulum (SR) is, in part, responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequstrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.