Thermo Fisher Scientific Vimentin Monoclonal Antibody (VI-01)

Thermo Fisher Scientific Vimentin Monoclonal Antibody (VI-01)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody reacts with vimentin, a 57 kDa intermediate filament intracellular protein expressed in a variety of mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscle desmin.

Immunocytochemistry:

• Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C
• Alternatively, fix directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C
• Positive controls: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line

Flow Cytometry:

• Suitable for intracellular staining

Target Information

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin is co-expressed with desmin during early cellular development and later replaced by tissue-specific IFPs. It also plays a role in steroid synthesis by facilitating cholesterol transport to mitochondria through cytoskeletal rearrangements induced by phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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