Thermo Fisher Scientific F13A1 Monoclonal Antibody (OTI3G12), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The F13A1 gene encodes the coagulation factor XIII A subunit, the final zymogen activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer (2 A subunits + 2 B subunits).

• A subunits: catalytic function
• B subunits: non-enzymatic, act as carrier molecules

Platelet factor XIII consists of two A subunits identical to plasma A subunits. Upon thrombin cleavage and calcium presence, the B subunits dissociate, producing active factor XIIIa, a transglutaminase that crosslinks fibrin molecules to stabilize clots. It also crosslinks alpha-2-plasmin inhibitor or fibronectin to fibrin alpha chains.

Factor XIII deficiency:

• Type I: lack of both A and B subunits
• Type II: lack of A subunit only
  These deficiencies can cause lifelong bleeding tendency, poor wound healing, and recurrent miscarriage.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.