Thermo Fisher Scientific Nestin Monoclonal Antibody (NES, 2911)
인간 Nestin 단백질을 인식하는 마우스 단클론 항체로, 줄기세포 및 신경세포 표지 연구에 적합합니다. 포르말린 고정 조직의 면역조직화학(IHC) 분석에 사용됩니다. 단백질 A/G로 정제된 액상 형태이며, 보존제 없이 PBS(pH 7.4)에 저장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NES, 2911 |
| Immunogen | Recombinant human NES protein fragment (aa 698–844) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C (preferred) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed):
Incubate antibody for 30 minutes at room temperature.
For formalin-fixed tissues, heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes.
Target Information
Nestin is a Class VI intermediate filament abundantly expressed in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. It serves as a key marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Nestin is also used as an angiogenic marker to evaluate neovascularity in tumors.
Highly specific antibodies to human Nestin assist in characterizing progenitor cells differentiating into distinct lineages and in studying the therapeutic potential of neural stem/progenitor cells for CNS diseases or injuries.
Nestin expression has also been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker in proliferating endothelial cells of colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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