Thermo Fisher Scientific CD33 Monoclonal Antibody (9A11-CD33), Super Bright 702, eBioscience

Thermo Fisher Scientific CD33 Monoclonal Antibody (9A11-CD33), Super Bright 702, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test
Publications: View 1 publication

Product Specifications

Additional Formats

• Alexa Fluor 647
• APC
• PE
• PE-Cyanine7
• Super Bright 436
• Request custom conjugation

Product Specific Information

This monoclonal antibody 9A11-CD33 reacts with mouse CD33 (Siglec3, gp67). CD33 is part of the sialic acid-binding immunoglobulin lectins (Siglec) family, binding sialic acid-containing glycans on mammalian cell surface glycoproteins and glycolipids. Mouse CD33 is expressed by mature granulocytes, immature myeloid cells, and microglia. Unlike human CD33, mouse CD33 lacks a cytoplasmic ITIM domain and exists as two splice variants with different cytoplasmic tails. In microglia, CD33 inhibits phagocytosis of toxic amyloid beta.

Clone 9A11 has been used to characterize mouse CD33 surface expression and function. View Article

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤ 0.25 µg/test (100 µL final volume). Optimal titration recommended.

Fluorochrome Information:
Super Bright 702 is a tandem dye excited by the violet laser line (405 nm) and emits at 702 nm. Recommended filter: 710/50 bandpass. Ensure instrument compatibility.

Staining Buffer Recommendation:
Use Super Bright Complete Staining Buffer (Product # SB-4401) when using multiple Super Bright dyes to minimize non-specific polymer interactions.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days at 4°C in the dark. Performance may vary by clone.

Excitation: 405 nm
Emission: 702 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD33 is a transmembrane protein of the Siglec family with ITIM motifs that recruit SHP-1 and SHP-2 phosphatases. It is expressed on myelomonocytic lineage cells and binds sialic acid residues in N- and O-glycans. CD33 is a therapeutic target for acute myeloid leukemia and is expressed on granulocyte and macrophage precursors, but not on pluripotent stem cells. It serves as a marker for peripheral monocytes and helps distinguish myelogenous leukemia cells from lymphoid or erythroid leukemias. Diseases linked to CD33 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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