Thermo Fisher Scientific Mouse IL-23 Recombinant Protein, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-Dependent

-

Functional Assay (Functional)

Assay-Dependent

-

Control (Ctrl)

Assay-Dependent

-

Miscellaneous PubMed (Misc)

-

View 9 publications 9 publications

Product Specifications

Species

Mouse

Published species

Not Applicable

Expression System

Insect cells

Amino acid sequence

Heterodimeric, mouse p40, amino acids Met1-Ser355 (Accession # NM_008352), and mouse p19, amino acids Met1-Ala196 (Accession # AF301619)

Molecular weight

55.6 kDa

Class

Recombinant

Type

Protein

Purity

>98% by SDS-PAGE

Endotoxin concentration

<0.01 ng/µg

Activity

ED50 < 625 pg/mL; as measured by the induction of IL-17A in mouse splenocytes.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

purified

Storage buffer

PBS, pH 6.0, with 1% BSA

Contains

no preservative

Storage conditions

-80°C

Shipping conditions

Dry ice

Product Specific Information

Description: IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-gamma. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-gamma production by naive and memory T cells, as compared to IL-12.

Applications Reported: Recombinant mouse IL-23 is biologically active.

Applications Tested: The ED50 of this protein, as measured by IL-17A induction in mouse splenocytes, is less than or equal to 625 pg/mL. This corresponds to a specific activity of greater than 1.5 x 10e6 Units/mg.

Source: Insect cells infected with baculovirus: mouse p40, amino acids met 1-Ser 355, accession # NM_008352 was co-expressed with mouse p19, amino acids met 1-Ala 196, accession # AF301619.

Bioactivity: The ED50 of this protein, as measured by IL-17A induction in mouse splenocytes, is less than or equal to 625 pg/mL. This corresponds to a specific activity of greater than 1.5 x 10e6 Units/mg.

Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.

Molecular Weight: The heterodimer of p40, amino acids met23-ser335, cystine-linked to p19, amino acids leu 20-ala 196, has a predicted molecular mass of 55,616. On non-reducing SDS-PAGE the heterodimeric cystine-linked protein migrates as a 60 kDa protein. The DTT reduced protein migrates as 43 kDa and 21 kDa polypeptides.

Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-γ. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naive and memory T cells, as compared to IL-12.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.