Thermo Fisher Scientific SHH Monoclonal Antibody (OTI10H6), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:2,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

Sonic Hedgehog (SHH) is expressed during embryogenesis and plays a crucial role in early embryo patterning. It acts as a key inductive signal for ventral neural tube formation, limb axis determination, and ventral somite development.
The SHH protein is synthesized as a precursor that undergoes autocatalytic cleavage. The N-terminal fragment is soluble and mediates signaling, while the C-terminal fragment aids in precursor processing. The C-terminal product attaches a cholesterol moiety to the N-terminal product, restricting its diffusion on the cell surface.
Defects in SHH or its signaling pathway can cause holoprosencephaly (HPE), characterized by forebrain malformation and facial deformities, and may be associated with VACTERL syndrome involving vertebral, anal, tracheoesophageal, renal, cardiac, and limb abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.