Thermo Fisher Scientific HDAC3 Polyclonal Antibody
HDAC3 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF, IP, ChIP 등 다양한 응용에 사용 가능. Human, Dog, Hamster, Rat 반응성. 항원 친화 크로마토그래피로 정제된 액상 항체. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 | - |
| Immunocytochemistry (ICC/IF) | 1:200 | - |
| Immunoprecipitation (IP) | - | View 1 publication |
| ChIP assay (ChIP) | 1–3 µL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Hamster, Human, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues CD(415)GDHDNDKESDVEI(428) of human HDAC3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2118393 |
Product Specific Information
PA1-862 detects histone deacetylase 3 (HDAC3) from canine, hamster, human, and rat tissues and cells.
It has been successfully used in Western blot and ChIP procedures.
By Western blot, this antibody detects an ~48 kDa protein representing HDAC3 from HeLa cell lysate.
The PA1-862 immunogen is a synthetic peptide corresponding to residues CD(415)GDHDNDKESDVEI(428) of human HDAC3.
This immunizing peptide (Cat. # PEP-071) is available for use in neutralization and control experiments.
Target Information
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events.
Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA.
HDAC3 belongs to the histone deacetylase/acuc/apha family.
It has histone deacetylase activity and represses transcription when tethered to a promoter.
HDAC3 may participate in transcriptional regulation through binding with the zinc-finger transcription factor YY1.
It can also down-regulate p53 function, thus modulating cell growth and apoptosis.
This gene is regarded as a potential tumor suppressor gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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