Thermo Fisher Scientific HDAC3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunocytochemistry (ICC/IF)

1:200

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

ChIP assay (ChIP)

1-3 µL

-

Product Specifications

Species Reactivity

Dog, Hamster, Human, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C D(415) G D H D N D K E S D V E I(428) of human HDAC3. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HDAC3,uniProtId:O15379-1,ncbiNodeId:9606,antigenRange:415-428,antigenLength:428,antigenImageFileName:PA1-862_HDAC3_O15379-1_Rabbit.svg,antigenImageFileNamePDP:PA1-862_HDAC3_O15379-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2118393

Product Specific Information

PA1-862 detects histone deacetylase 3 (HDAC3) from canine, hamster, human and rat tissues and cells.

PA1-862 has been successfully used in Western blot and ChIP procedures. By Western blot, this antibody detects an ~48 kDa protein representing HDAC3 from HeLa cell lysate.

The PA1-862 immunogen is a synthetic peptide corresponding to residues C D(415) G D H D N D K E S D V E I(428) of human HDAC3. This immunizing peptide (Cat. # PEP-071) is available for use in neutralization and control experiments.

Target Information

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC3 belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.