Thermo Fisher Scientific Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
HRP 결합형 Donkey anti-Goat IgG (H+L) 2차 항체로, Western blot, IHC, ELISA 등 다양한 면역 실험에 적합합니다. 사람, 토끼, 생쥐 등 타종 IgG에 대한 교차 흡착 처리로 높은 특이성과 낮은 비특이적 결합을 제공합니다. 리오필라이즈드 형태로 안정적이며 재구성 후 -20°C 보관 권장.
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Thermo Fisher Scientific Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:100,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:5,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:500–1:5,000 |
| ELISA | 1:5,000–1:100,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Goat |
| Host/Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Proprietary Immunogen |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1.5% BSA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_933637 |
| Target | IgG |
| Cross Adsorption | Against human, rabbit, rat, horse, guinea pig, hamster, chicken, and mouse IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
- Store at 4°C prior to reconstitution.
- After reconstitution, store at -20°C.
- For long-term storage, addition of 0.01% thiomersal is recommended.
- Reconstitute with 0.5 mL of distilled water.
- Donkey anti-Goat IgG antibody recognizes goat immunoglobulin G and has been absorbed against multiple species to minimize cross reactivity.
Target Information
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins. They are used for detection, sorting, or purification of target proteins.
Secondary antibodies provide versatility and sensitivity by enabling multiple detection systems such as HRP, AP, or fluorescence, and enhance signal amplification through multiple binding events.
Typically, these antibodies are generated by immunizing host animals with pooled immunoglobulins from the target species and purified via immunoaffinity chromatography or conjugation techniques to produce highly specific reagents.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
Available Conjugate Formats
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 647, 680
- AP, Biotin, DSB-X Biotin
- DyLight 350, 488, 550, 594, 650, 680, 755, 800
- FITC, TRITC
- Custom conjugation available upon request
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