Thermo Fisher Scientific ERCC1 Monoclonal Antibody (OTI1A3), TrueMAB
ERCC1 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 사용 가능. 인간 시료 반응성. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도 유지. DNA 손상 복구 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A3 |
| Immunogen | Full-length protein expressed in 293T cells transfected with human ERCC1 expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
ERCC1 is a non-catalytic component of a DNA repair endonuclease responsible for the 5′-incision during DNA repair. Together with SLX4, it initiates repair of interstrand cross-links (ICL) and participates in processing anaphase bridge-generating DNA structures that can lead to cytokinesis failure. ERCC1 also functions in homology-directed repair (HDR) of DNA double-strand breaks in conjunction with SLX4. (Uniprot)
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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