Thermo Fisher Scientific CA9 Monoclonal Antibody (OTI1G7), TrueMAB
CA9 단백질을 인식하는 Mouse Monoclonal Antibody (Clone OTI1G7). Western blot, IHC, Flow cytometry에 적합. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도. CA IX는 저산소 상태에서 발현되는 세포막 단백질로, 저산소 마커 연구에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G7 |
| Immunogen | Full-length protein expressed in 293T cell transfected with human CA9 expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Carbonic anhydrase (CA) is an enzyme that catalyzes the rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions.
It is widely expressed in mammalian tissues and regulated by hypoxia through HIF-1α.
CA IX, a membrane-associated isoform, is a highly inducible gene under hypoxic conditions and serves as a reliable histochemical marker of hypoxia.
Inhibition of CA IX increases cell death under hypoxia, indicating its role in pH regulation and cell survival.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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