
Thermo Fisher Scientific F(ab`)2-Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody
Thermo Fisher Scientific의 F(ab`)2-Rabbit anti-Mouse IgG (H+L) 항체는 비결합형 2차 항체로, 마우스 IgG 검출용으로 최적화되어 있습니다. IHC 및 ELISA에 적합하며, 인간 혈청 단백질에 대한 교차 반응이 최소화되었습니다. 연구용으로 고순도, 고특이성 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 – 1:500 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100 – 1:500 |
| ELISA | 1:500 – 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Whole mouse IgG |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C or -20°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2550671 |
| Target | IgG |
| Cross Adsorption | Against human serum proteins |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
F(ab`)2 fragments were prepared by pepsin digestion of IgG followed by gel filtration to remove intact IgG or Fc fragments.
This antibody cross-reacts with rat samples and has minimized cross-reactivity with human serum proteins through solid phase adsorption.
Suitable as a bridge in a PAP/APAAP complex.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins.
They are useful for detection, sorting, or purification of mouse IgG and offer increased versatility for various detection systems (HRP, AP, fluorescence).
Secondary antibodies enhance sensitivity through signal amplification since multiple secondary antibodies can bind to a single primary antibody.
They are typically generated by immunizing host animals with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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