Thermo Fisher Scientific HTR1B Polyclonal Antibody

Applications and Tested Dilution

• Immunohistochemistry (Paraffin) [IHC (P)]: 10 µg/mL

Product Specifications

Product Specific Information

Percent identity with other species by BLAST analysis:

• 100%: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset
• 94%: Mouse, Rat, Bat, Horse, Pig
• 88%: Hamster, Panda, Bovine, Dog, Cat, Rabbit, Guinea pig
• 82%: Elephant, Opossum

For IHC(P), use proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Target Information

Serotonin (5-hydroxytryptamine, 5-HT) is a serum factor that regulates diverse biological processes in the central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems.
Molecular cloning studies have identified five receptors belonging to the 5-HT1 subtypes, which are negatively coupled to adenylyl cyclase.
Radioligand binding studies indicate the presence of 5-HT1B (formerly 5-HT1DbR) sites in the substantia nigra, dorsal subiculum, globus pallidus, and superior colliculus.
In situ hybridization studies show abundant 5-HT1BR mRNA in CA1 pyramidal cells of the hippocampus, Purkinje cells of the cerebellum, olfactory tubercle, subthalamic nucleus, and lower levels in the caudate-putamen, cortical areas, thalamic, and hypothalamic nuclei.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.