Thermo Fisher Scientific PDE3B Monoclonal Antibody (SMCP3B), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

SMCP3B

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1603298

Product Specific Information

Description: Cyclic nucleotide phosphodiesterases (PDE) catalyze the hydrolysis of cAMP and cGMP thereby regulating the effects of these second messengers. There are several families of PDE which share common structural organization. The PDE3 family has two members, 3A and 3B that are products of two separate genes. PDE3B (130 kDa) is expressed predominantly in adipose tissue and lymphocytes, while PDE3A (120 kDa) is expressed predominantly in platelets and heart tissue.

The monoclonal antibody SMCP3B reacts with mouse PDE3B. The SMCP3B antibody was generated against amino acids 643-1100 in the C-terminus and does not cross-react with mouse PDE3A, as determined by western blot analysis of lysates of mouse heart tissue. The SMCP3B antibody does not cross-react with human PDE3B.

Applications Reported: This SMCP3B antibody has been reported for use in immunoblotting (WB).

Applications Tested: This SMCP3B antibody has been tested by immunoblotting of mouse lymph node cells and mouse heart under reducing conditions. It can be used at 1-5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.